INSTITUTE OF BIOPHYSICS; CHINESE ACADEMY OF SCIENCES
发明人:
MA, Yue,马跃
申请号：
CNCN2013/079811
公开号：
WO2014/015777A1
申请日:
2013.07.22
申请国别(地区):
WO
年份:
2014
代理人:
摘要：
Provided in the present invention is a method for inducing pluripotent stem cells to differentiate into ventricular myocytes in vitro, which is achieved by maintaining, amplifying and culturing pluripotent stem cells in vitro, adding a substance capable of activating the Smad1/5/8 signaling pathway directly or indirectly into the culture medium when pluripotent stem cells are in the middle stage of myocardial differentiation, i.e. the period of differentiating into cardiac muscle cells from mesoderm cells or myocardial precursor cells, which enables stem cells to differentiate into ventricular myocytes directionally. Ventricular myocytes with biological activity and function are obtained successfully by means of the method of the present invention, which reveals the regulatory mechanism during differentiation of myocardial precursor cells into ventricular myocytes moreover, the human ventricular myocytes obtained via differentiation can be widely used in treating myocardial infarction by cell transplantation, in toxicological analysis of the heart and in the development of heart-related drugs.Cette invention concerne une méthode permettant dinduire la différenciation de cellules souches pluripotentes en myocytes ventriculaires in vitro, ladite méthode consistant à conserver, à amplifier, à cultiver les cellules souches pluripotentes in vitro, à ajouter une substance capable dactiver la voie de signalisation Smad1/5/8 directement ou indirectement dans le milieu de culture lorsque lesdites cellules souches pluripotentes se trouvent dans la phase intermédiaire de la différenciation myocardique, autrement dit la phase où les cellules du muscle cardiaque se différencient à partir des cellules du mésoderme ou des cellules précurseurs myocardiaques, ce qui permet aux cellules souches de se différencier en myocytes ventriculaires de manière directionnelle. La méthode selon linvention permet dobtenir avec succès des myocytes ventriculaires doués dactivité et de fonction biol
