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НОВЫЙ ИММУНОГЕННЫЙ ЭКСТРАКТ ГРИБОВ И ПАТТЕРН-РАСПОЗНАЮЩИЙ РЕЦЕПТОР РАСТЕНИЙ
专利权人:
ЭБЕРХАРД КАРЛС УНИВЕРЗИТЭТ ТЮБИНГЕН (DE)
发明人:
ГУСТ Андреа (DE),БРУННЕР Фредерик (DE),ФРАИТУР Малоу (LU),ЧЖАН Вэйго (CH)
申请号:
RU2015111351
公开号:
RU2015111351A
申请日:
2013.10.22
申请国别(地区):
RU
年份:
2016
代理人:
摘要:
1. An immunogenic against plant fungi extract which is prepared by a method comprising the following steps: (a) providing a culture filtrate of cells Sclerotinia sclerotiorum (Sclerotinia sklerotsievidnoy), (b) introducing said filtrate from part (a) in the first cation exchange column equilibrated with low salt. buffer a (c) elution of high salt buffer extract B (g) diluting the eluted fraction of n. (c) a buffer enabling binding to implement the second cation exchange column, (d) introducing the diluted fraction of n. (g) in sec w cation exchange column and eluting the extract buffered saline, whose conductivity of 5-20 mS / cm, preferably 8-16 mS / see 2. The immunogenic against fungal plant extract according to Claim. 1, where buffer A was 100 mM Mes buffer pH 5.4 and / or the buffer B is a 100 mM Mes buffer pH 5.4, 500 mM KCl.3. The immunogenic against fungal plant extract according to Claim. 1 or 2, wherein said first cation exchange column comprises a sorbent GE Healthcare Sepharose SP FastFlow.4. The immunogenic against fungal plant extract according to any one of claims. 1 or 2, wherein said second cation exchange column is a GE Healthcare Source 15S 4.6 / 100 PE kolonku.5. The immunogenic against fungal plant extract according to any one of claims. 1 or 2, wherein in step (g) eluted fraction is diluted with buffer A at a ratio of 1 to 10.6. The immunogenic against plant fungi extract containing at least one of the proteins selected from Cytochrome C (A7E6R4), N-acetyltransferase (A7F941), dissociation inhibitor of Rho-GDP (A7ET57) polyubiquitin (A7E4E9), protein disulfide isomerase (A7EDH2 ) or a pectin esterase (A7EXV0) .7. The immunogenic against fungal plant extract according to claim. 6, which comprises at least two of these proteins, preferably, three of these proteins1. Иммуногенный в отношении растений экстракт грибов, который получают способом, включающим следующие стадии:(а) предоставление культурального фильтрата клеток Sclerotinia sclerotiorum (
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