The Broad Institute Inc.;Massachusetts Institute of Technology;Presidents and Fellows of Harvard College
发明人:
Feng ZHANG,Patrick HSU,Chie-yu LIN,Fei RAN
申请号：
US14972523
公开号：
US20160153006A1
申请日:
2015.12.17
申请国别(地区):
US
年份:
2016
代理人:
摘要：
The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
