PROBLEM TO BE SOLVED: To provide a method for culturing Basidiomycetes that can securely prevent microbial contamination by a simple step, omitting the sterilization step and the inoculation/initial culture step in a clean room in the mushroom cultivation.SOLUTION: The method is characterized in that a Streptomycete and a Basidiomycete are inoculated to a mixture in an ordinary room, and cultured at 3-12°C. The mixture is prepared by mixing a culture bed and nutrients with moisture control. The Streptomycete is Streptomyces rimosus. The Streptomycete and the Basidiomycete are mixed into the culture bed and then cultured.COPYRIGHT: (C)2014,JPO&INPIT【課題】キノコ栽培における殺菌工程と、無菌室での接種・初期培養工程を省き、簡単な工程で確実に雑菌の繁殖を抑えることができる担子菌類培養方法を提供する。【解決手段】培地基材と栄養剤を混合して水分調整して得られる混合物に、通常の室内で放線菌Streptomyces属菌と担子菌を接種し、3~12℃で担子菌類を培養する。放線菌Streptomyces属菌は、Streptomyces rimosusである。放線菌Streptomyces属菌と担子菌とを、培地基材とともに混合して培養するものである。【選択図】図1