The present invention discloses for providing a composite seal film on an opening at a top end of a culture container to secure with a cover of the culture container rotarily and there is at least one vent on the culture container or the cover of the culture container in predetermined positions. An upper flat of the cover of the culture container is covered a first airproof substrate film by heat press and a bacterioseitic layer is spreading on the first substrate film and then; a second airproof substrate film is covered on a top surface of the bacterioseitic layer by heat press so as to let the upper flat of the cover of the culture combine with the composite seal film completely. Before covered, the first and second substrate films of the composite seal film form a plurality of microgap structure regions. Accordingly, the culture container can provide a well environment having well ventilation without bacteria for plants via the microgap structure regions of the composite seal film and the inside of the culture container becomes a well environment having well intensity of illumination; humidity and temperature.本發明是提供一種培養容器之封口膜組成,是在培養容器的上端開口為配合一容器蓋作方位旋鎖,而該容器或容器蓋在預設方位是設有至少一道以上的透氣孔,在該容器蓋的上端層面為藉熱壓來覆設一不透氣的第一基材膜,及沿著第一基材膜上是可塗覆以一層抑殺菌劑層,再由該抑殺菌劑層上端則在藉熱壓來覆設以具不透氣的第二基材膜,以使容器蓋上端是結合有一封口膜,而該封口膜的第一、第二基材膜在覆設之前是成形有所須範圍、及大小的多數個微觀間隙結構(microgap structure)區域;依此,作為在植入種苗後能藉容器蓋其間的封口膜來獲有供給一種苗成長所需無菌良好空氣對流空間之培養容器,其內部同時具有極佳之光線照度、溼度及溫度環境。