A dental floss dispensing assembly includes a housing that includes a top wall, a bottom wall and a perimeter wall which is attached to and extends between the top and a bottom walls. The top wall is convexly arcuate and has a domed shape. The perimeter wall tapers outwardly from the top wall to a central area of the housing and tapers inwardly from the central area to the bottom wall such that the perimeter wall is egg-shaped. A support is mounted within the interior of the housing and retains a spool holding dental floss such that the spool is rotatable with respect to the housing. The housing has an aperture extending therethrough and the dental floss is extendable through the aperture.