A respiratory training assembly has a main body, an expiratory training device mounted in the main body and a handle formed on the main body. The main body has a volume barrel, an inspiratory training device, an air channel and an output-input entrance. The inspiratory training device is mounted movably in the volume barrel and has a floating element. The air channel is formed on the main body, communicates with the volume barrel, and has a check valve. The output-input entrance is formed in the main body and communicates with the air channel. In use, the inspiratory flow path and the expiratory flow path of the respiratory training assembly are separated to prevent pollution from the patient's saliva. Additionally, the expiratory training device can be adjusted according to the patient's requirement to improve the training result.
