The present invention relates to the preparation and use of a cell cryopreservation formulation convenient for clinical use. In particular, provided is a cell cryopreservation (freezing) formulation, comprising: (1) a cell； (2) a cryopreservation diluent, the cryopreservation diluent comprising: an aqueous solution of sodium chloride, protective proteins, and dimethyl sulfoxide. The present invention also involves a corresponding cell recovery method. The present invention uses a cryopreservation media requiring no washing to prepare a cell cryopreservation formulation, and cell recovery and dilution for clinical use can be performed by means of a simple preparation operation.
