This invention relates to the use of the combination of 25-hydroxyvitamin D3 (“25-OH D3”) and antioxidants/anti-inflammatories (ascorbic acid, vitamin E and at least one carotenoid) to make a pharmaceutical, nutraceutical or food supplement which can ameliorate various problems observed in bovines connected cystic ovarian disease, or increasing 17-β estradiol levels. Feed and premixes containing the 25-OH D3 and antioxidants/anti-inflammatories and premixes are also provided.