Bennett, Robert P.,Chesnut, Jonathan D.,Heyman, John,Madden, Knut R.,Shuman, Stewart
申请号：
AU2012200337
公开号：
AU2012200337A1
申请日:
2012.01.20
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
#$%^&*AU2012200337A120120209.pdf#####ABSTRACT The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3 terminus of one end and has a single stranded 5 overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5 overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practising the methods, including methods of directionally linking two or more ds nucleic acid molecules,
