An improved isolator for collection, parametrical characterization and long-term preservation of samples containing stem cells comprises: a work chamber (2) isolated from the external environment gloves jutting out at an inside of said work chamber (2) an inlet chamber (4) for introduction of a sample to be processed an outlet chamber connected with the work chamber (2) and having a sterile sample-collecting bag sterilizing means acting on said sample and/or on said inside of the work chamber (2) filtering means cooling means for bringing and keeping said sample at a constant working temperature throughout the inlet chamber (4), the work chamber (2) and the outlet chamber and visual imaging means (5) focused on the sample and defining a visual path which maintains a complete mechanical and pneumatic/hydraulic isolation between said inside of the work chamber (2) and the external environment.
