The invention of a production device for queen bees and royal jelly comprises a spawn-incubating unit (20) including spawn-incubating plates (21, 22), worker bee inlets (23) and a queen bee inlet (24) on the plates (21, 22), spawning holes (25) formed on a side of the inlets (23, 24) in which the queen bee can spawn, and feed chambers (32) formed as separate chambers at a side of the inner space (28) and communicating with the inner space (28) or formed on the inner side of the plates (21, 22) as a plurality of recesses, in which feeds such as pollen, water and sugar solution are supplied a plurality of larva cell pieces (41) or a larva cell strip (40) and a production unit (50) which includes a plurality of queen houses (60) or a queen house strip (52).
