Disclosed are compositions enriched with thymohydroquinone, further comprising of thymoquinone, hederagenm and/or a-hederin formulated by blending the active molecules isolated from the seeds of Nigella sativa. Also disclosed are novel processes for the isolation of bioactive components thymohydroquinone, thymoquinone from the seeds of Nigella sativa. A process for the isolation a-hederin and hederagenin from the spent material of Nigella sativa is also disclosed herein.