A living cell cryopreservation tool (1) has a body part (3) formed of a cold-resistant material and a living cell holding part (2) formed of the cold-resistant material. The living cell holding part (2) has a long and narrow living cell attaching and holding portion (22). The living cell attaching and holding portion (22) has a plurality of living cell accommodation concave portions (28a, 28b, 28c, 28d, and 28e) formed in a longitudinal direction thereof and a plurality of excess cryopreservation liquid discharge passages (29a, 29b, 29c, 29d, 29e, and 29f) communicating with the living cell accommodation concave portions (28a, 28b, 28c, 28d, and 28e).