A manufacturing method of a bioactive composition, which contains at least a bioactive component, includes steps of preparing a first solution having a first temperature preparing a second solution containing said bioactive component and having a second temperature lower than the first temperature mixing the first and the second solutions to produce a third solution adding at least a starter culture to the third solution in a temperature of 20~60℃ preserving the third solution in said temperature decreasing the temperature of the third solution to no greater than 20℃. A bioactive composition made by the above-mentioned manufacturing method is also provided.一種含有至少一具有生物活性之組分之生物活性組合物的製造方法,係包含下列步驟:製備第一混合液,具有第一溫度;製備第二混合液,含有該具有生物活性之組分,具有低於第一溫度之第二溫度;混合第一混合液與第二混合液,得到第三混合液;在第三混合液為20~60℃之溫度條件下,加入至少一菌元;保存第三混合液於大於20℃及小於60℃之溫度;降低第三混合液之溫度至小於等於20℃。一種生物活性組合物,由上述製造方法製成。1...製備第一混合液2...製備第二混合液3...混合第一混合液與第二混合液4...加入菌元與進行發酵反應5...結束發酵反應與儲存6...測試微生物或具生物活性之組分
