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POPULATIONS OF REPORTER SEQUENCES AND METHODS OF THEIR USE
专利权人:
ATTAGENE; INC.
发明人:
ROMANOV, SERGEI,MAKAROV, SERGEI
申请号:
CA2587338
公开号:
CA2587338C
申请日:
2005.11.10
申请国别(地区):
CA
年份:
2016
代理人:
摘要:
Compositions, methods and kits are provided that are useful, for example, fordetermining activities of multiple cis-regulatory sequences, such as promotersand enhancers, and/or multiple trans-acting factors, such as transcriptionfactors, in a cell. In particular, in certain embodiments, compositions areprovided comprising a population of polynucleotide reporter transcriptionunits (RTUs) in which each RTU comprises a reporter sequence, a processing taglocated in the reporter sequence and a cis-regulatory element operably linkedto the reporter sequence, wherein the reporter sequences between any two RTUsin the population, outside of the processing tags, are substantially identicaland wherein the positions of the processing tags within the reporter sequencesdistinguish between any two RTUs differing, for example, in their cis-regulatory elements. The compositions, methods and kits can further be used,for example, to identify a cell type or disease state, for example, in abiological organism.
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中国工程科技知识中心
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