SEATTLE CHILDREN'S HOSPITAL, DBA SEATTLE CHILDREN'S RESEARCH INSTITUTE;FRED HUTCHINSON CANCER RESEARCH CENTER
发明人:
RIDDELL, STANLEY R.,HUDECEK, MICHAEL,JENSEN, MICHAEL
申请号：
CA2881981
公开号：
CA2881981A1
申请日:
2013.08.20
申请国别(地区):
CA
年份:
2014
代理人:
摘要：
The present invention provides nucleic acids, vectors, host cells, methods and compositions to confer and/or augment immune responses mediated by cellular immunotherapy, such as by adoptively transferring CD8+ central memory T cells or combinations of central memory T cells with CD4+ T cells that are genetically modified to express a chimeric receptor. In embodiments the genetically modified host cell comprises a nucleic acid comprising a polynucleotide coding for a ligand binding domain, a polynucleotide comprising a customized spacer region, a polynucleotide comprising a transmembrane domain, and a polynucleotide comprising an intracellular signaling domain. It has been surprisingly found that the length of the spacer region can affects the ability of chimeric receptor modified T cells to recognize target cells in vitro and affects in vivo efficacy of the chimeric receptor modified T cells. Pharmaceutical formulations produced by the method, and methods of using the same, are also described.
