METHODS OF PREPARING PLANT-DERIVED VIRUS LIKE PARTICLES (VLPS) ARE PROVIDED. THE METHOD MAY COMPRISE OBTAINING A PLANT, OR PLANT MATTER COMPRISING APOPLAST-LOCALIZED VLPS, PRODUCING A PROTOPLAST/SPHEROPLAST FRACTION AND APOPLAST FRACTION FROM THE PLANT OR PLANT MATTER, AND RECOVERING THE APOPLAST FRACTION. THE APOPLAST FRACTION COMPRISES PLANT-DERIVED VLPS. ALTERNATIVELY, VLPS MAY BE OBTAINED FROM PLANT OR PLANT MATTER COMPRISING PLANT-DERIVED VLPS BY DIGESTING THE PLANT MATTER USING A CELL WALL DEGRADING ENZYME COMPOSITION TO PRODUCE A DIGESTED FRACTION. THE DIGESTED FRACTION IS FILTERED TO PRODUCE A FILTERED FRACTION, AND THE PLANT-DERIVED VLPS ARE RECOVERED FROM THE FILTERED FRACTION.
