With what by modifying in the method for natural 7 virus of ECHO to prepare modified 7 type enterovirus of ECHO,Wherein 7 virus of natural ECHO separates Bing by known method from human faecal mass and is identified by genome sequence,The modification is following to be carried out: viral adaptation is carried out first in the cancer cell weakened by anticancer agent Dacarbazine,Modified virus is further passed in human embryonic fibroblast's culture,It breeds in human melanoma cell with Hou and is further passed in the human embryonic fibroblast's culture handled by Ribavirin,It is separated and is purified by known method. Modified virus is suitble to treat various tumours with what.在用於通過修飾天然ECHO 7病毒來製備經修飾的ECHO 7型腸道 病毒的方法中，其中所述天然ECHO 7病毒通過已知方法從人糞 便中分離並通過基因組序列鑒定，所述修飾如下進行：首先在通過抗癌劑達卡巴嗪弱化的癌細胞中進行病毒適應，進一步在人胚 胎成纖維細胞培養物中傳代經修飾的病毒，隨後在人黑色素瘤細胞中繁殖並進一步在通過利巴韋林處理的人胚胎成纖維細胞培養 物中傳代，通過已知方法分離和純化。經修飾的病毒適合用於治 療各種腫瘤。
