The invention provides a virus strain capable of being used for preparing a VII type Newcastle disease vaccine and an encoding gene thereof. The virus strain is named Genotype VII Newcastle disease virus (VII NDV)/TW-CB-ND02 with the accession number of BCRC 970070, VII NDV/TW-CB-ND04 with the accession number of BCRC 970071, or VII NDV/TW-CB-ND06 with the accession number of BCRC 970072. The virus strain can effectively improve immunity of a host to Newcastle disease viruses and produce antibody immune sustaining force in a poultry body, and is applicable to research, development and preparation of a Newcastle disease vaccine; moreover, it can also be used for manufacturing an activated, an inactivated or attenuated type vaccine.本發明為一種適用於Ⅶ型新城病疫苗之病毒株及其編碼基因,其係該病毒株之生物寄存號碼為BCRC 970070、名稱為禽類七型新城病病毒(Genotype VII Newcastle disease virus)VII NDV/TW-CB-ND02的病毒株;生物寄存號碼為BCRC 970071、名稱為禽類七型新城病病毒VII NDV/TW-CB-ND04的病毒株;或生物寄存號碼為BCRC 970072、名稱為禽類七型新城病病毒VII NDV/TW-CB-ND06的病毒株。該病毒株具有符合能夠有效提高宿主對新城病病毒的免疫力反應、及在禽類體內產生抗體免疫持續力之防疫功效,適合於生產預防新城病疫苗的研究及開發,也可用於製造活毒型、去活化型或減毒型疫苗。