The purpose of the present invention is to obtain an emission image without causing reduction in signal-to-noise ratio of the emission image even when the emission wavelength from a light emitting agent belongs to a visible light band. An image acquisition system according to the present disclosure includes: a first narrow band light source (101) that emits first narrow band light for exciting a light emitting agent that is present in a subject to be observed and emits light at a wavelength that belongs to a visible wavelength band； a second narrow band light source (103) that emits second narrow band light that has a wavelength band in a range of ±30 nm of the emission peak wavelength of the light emitting agent； a wide band light source (105) that emits wide band light for illuminating the subject to be observed； a first imaging element (201) at which light of an emission wavelength band that includes a wavelength corresponding to light emitted from the light emitting agent forms an image； and a second imaging element (203) that is composed of one or a plurality of imaging elements at which light of wavelength bands other than the emission wavelength band is forms an image. A first captured image obtained from the first imaging element and a second captured image obtained from the second imaging element are synthesized to create a captured image relating to the subject to be observed.L'objectif de la présente invention est d'obtenir une image d'émission sans provoquer de réduction du rapport signal sur bruit de l'image d'émission même lorsque la longueur d'onde d'émission d'un agent électroluminescent appartient à une bande de lumière visible. Selon la présente invention, un système d'acquisition d'image comprend : une première source de lumière à bande étroite (101) qui émet une première lumière à bande étroite afin d'exciter un agent électroluminescent qui est présent dans un sujet à observer et émet de la lumière à une longueur d'onde qui appartient à une bande
