1. A method for the preparation and purification of wild-type orthopoxvirus, attenuated and / or recombinant orthopoxvirus, comprising the following steps: a) preparing a culture of packaging cells； b) infection of the culture of packaging cells with orthopoxvirus； c) culturing infected packaging cells to produce offspring of orthopoxvirus； d) incubation in the presence of one or more nucleases； e) isolation of orthopoxviruses from the culture supernatant and / or packaging cells； f) addition of monovalent salts to the orthopoxviruses isolated in step e) under conditions x, suitable for inhibiting the activity of nuclease (nucleases) and for preventing the adsorption of the indicated orthopoxviruses to the anion exchange adsorbent in step g)； g) contacting the mixture obtained in step f) with the anion exchange adsorbent under conditions suitable for capture of nucleic acids； h) clarification the mixture obtained in step g) under conditions suitable for removing cell debris； i) washing the anion exchange adsorbent with a solution containing monovalent salts under conditions suitable for isolating the remaining orthopoxviruses filtrate； j) concentrating the filtrate obtained in step h), and the filtrate obtained in step i)； k) diafiltration orthopoxvirus containing fraction obtained in step j) .2. The method of claim 1, wherein the packaging cells are immortalized cell lines, preferably immortalized bird cell lines. The method of claim 2, wherein the immortalized bird cell lines are capable of growing in suspension without (micro) carriers, said cells being preferably cultured in an environment free of animal products. Spo1. Способ получения и очистки ортопоксвируса дикого типа, аттеньюированного и/или рекомбинантного ортопоксвируса, включающий следующие этапы:a) приготовление культуры пакующих клеток；b) инфицирование культуры пакующих клеток ортопоксвирусом；c) культивирование инфицированных пакующих клеток до получения потомства ортопоксвируса；d) инкубация в присутс
