PROBLEM TO BE SOLVED: To provide a puncture device capable of easily embedding a bio-tissue support indwelling object in a living body, imposing a reduced burden on a patient, and providing the patient with high safety, and a puncture method.SOLUTION: A puncture device 1 includes: contact parts 221 and 222 that come into contact with a living body surface a puncture member 3 that rotationally turns about a turning axis, with the contact parts 221 and 222 coming into contact with the living body surface, and punctures a bio-tissue a urethra insertion part 41 to be inserted into the urethra and a frame 2 that regulates a physical relationship among the contact parts 221 and 222, the puncture member 3, and the urethra insertion part 41.COPYRIGHT: (C)2015,JPO&INPIT【課題】生体組織支持用留置物を容易に生体内に埋設することができ、かつ、患者の負担が少なく、患者の安全性が高い穿刺装置および穿刺方法を提供すること。【解決手段】穿刺装置1は、生体表面と当接する当接部221、222と、当接部221、222が生体表面に当接した状態で、回動軸回りに回動し、生体組織を穿刺する穿刺部材3と、尿道に挿入される尿道挿入部41と、当接部221、222、穿刺部材3、穿刺部材3および尿道挿入部41の位置関係を規制するフレーム2とを有する。【選択図】図2