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Methods and compositions for preparation of nucleic acids
专利权人:
Syngenta Participations AG
发明人:
Ji, Yanshan,Wang, Wenling,Huang, Er-Te Jamie,Moore, Karen Elizabeth,White, Kimberly Ann
申请号:
AU2014221333
公开号:
AU2014221333A1
申请日:
2014.02.10
申请国别(地区):
AU
年份:
2015
代理人:
摘要:
A method for isolating genomic DNA (gDNA) from a biological material, the method including (a) contacting a sample that contains gDNA with a solution of hydroxide and a detergent to denature the gDNA (b) mixing into the solution resulting from step (a) a solution characterized by high salt and reducing the pH of the solution to less than 1 0, thereby producing a neutralized solution (c) centrifuging the sample to clarify the preparation and (d) removing Insoluble material from the neutralized and clarified preparation, whereby a solution of gDNA is produced.
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