JI, Yanshan,WANG, Wenling,HUANG, Er-Te Jamie,MOORE, Karen Elizabeth,WHITE, Kimberly Ann
申请号：
EP14756408
公开号：
EP2961264A4
申请日:
2014.02.10
申请国别(地区):
EP
年份:
2016
代理人:
摘要：
A method for isolating genomic DNA (gDNA) from a biological material. In some embodiments, the method includes (a) contacting a sample that contains gDNA with a solution of hydroxide and a detergent under conditions and for a time sufficient to degrade a cell wall, a cell membrane, a nuclear membrane, a nucleoprotein, or combinations thereof and/or to denature the gDNA (b) mixing into the solution resulting from step (a) a solution characterized by high salt and sufficient buffering capacity to reduce the pH of the solution to less than 10, thereby producing a neutralized solution (c) centrifuging the sample at a speed and length of time sufficient to clarify the preparation and (d) removing insoluble material from the neutralized and clarified preparation, whereby a solution of gDNA is produced. Also provided are method for performing a quantitative polymerase chain reaction (qPCR) of a gDNA sample, methods for performing genotyping or other molecular marker analysis of a gDNA sample, and methods for determining a haplotype of a cell.
