The present invention relates to the development of a novel clone of Vetiver [Vetveria zizaniodes (L) Nash. syn. Chrysopogon zizaniodes (L.) Roberty family Poaceae], named ‘CIMAP-KHUS 40’ characterised by somatic chromosome number 4x=40, larger stomata, fast growing deep penetrating roots, and seed infertility disabling its spread as a weed. This clone has unique ISSR and RAPD profiles that serve as DNA-fingerprints, and is developed from a unique diploid plant (2n=20). The invention document details all the pertinent data relating to this clone, its biological features and usefulness, and the method of its development.