ＥＳ細胞の継代方法及びＥＳ細胞増殖方法

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专利权人:

发明人:

申请号：

JP20090171639

公开号：

JP5750793(B2)

申请日:

2009.07.22

申请国别(地区):

日本

年份:

2015

代理人:

摘要：

PROBLEM TO BE SOLVED: To provide a cell adsorbent that strongly attaches or adsorbs various cells, a cell culture substrate, a method for producing the same, a cell passage method, and a cell proliferation method.

SOLUTION: The cell culture substrate includes a cell adsorbent composed of at least one kind selected from animal-derived fibrous or filmy natural organic polymers chemically modified and processed or graft processed. The cell culture substrate is produced by immersing the natural organic polymer in a graft processing liquid comprising a surfactant aqueous solution and a monomer containing fluorine, an aqueous solution containing a phosphoric monoester, methacrylamide and 2-hydroxyethyl methacrylate or an organic solvent solution of a dibasic acid anhydride or epoxy compound and performing graft processing or chemical modification processing in the presence of a polymerization initiator. Cells are passaged using the cell culture substrate in an undifferentiated state in a proliferative env

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