PROBLEM TO BE SOLVED: To provide a method for controlling specific differentiation from a pluripotent cell such as a non-embryonic human artificial pluripotent stem cell (hiPSC) and a human embryonic stem cell (hESC) to a mesencephalon fate FOXA2 positive LMX1A positive TH positive dopamine (DA) neuron.SOLUTION: A method for exposing a pluripotent cell to a) one or more SMAD signal transduction inhibitors, b) one or more SHH signaling activators, and c) one or more Wnt signaling activators； and exposing one or more Wnt signaling activators to the pluripotent cell at effective concentration for 3 days from the start of contact with one or more SMAD signal transduction inhibitors such that the pluripotent cell is differentiated to create a plurality of cells expressing both FOXA2 and LMX1A, in which an obtained DA neuron is rich in markers such as CD142 and A9-type neurocyte.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2018,JPO&INPIT【課題】非胚性ヒト人工万能性幹細胞（ｈｉＰＳＣ）、ヒト胚性幹細胞（ｈＥＳＣ）等の万能細胞から中脳運命ＦＯＸＡ２陽性ＬＭＸ１Ａ陽性ＴＨ陽性ドーパミン（ＤＡ）ニューロンへの特異的分化を制御する方法の提供。【解決手段】万能性細胞をａ）１つ以上のＳＭＡＤシグナル伝達阻害剤ｂ）１つ以上のＳＨＨシグナル伝達活性化剤及びｃ）１つ以上のＷｎｔシグナル伝達活性化剤、に暴露すること；前記万能性細胞が分化してＦＯＸＡ２とＬＭＸ１Ａの両方を発現する複数の細胞を生成するように、１つ以上のＷｎｔシグナル伝達活性化剤を、１つ以上のＳＭＡＤシグナル伝達阻害剤との接触開始から３日間有効濃度で前記万能性細胞に暴露し、得られたＤＡニューロンはＣＤ１４２などのマーカー及びＡ９型神経細胞に富む方法。【選択図】図１
