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METHOD FOR PRODUCING HIGH-QUALITY RECOMBINANT ALLERGENS IN A PLANT
专利权人:
ANGANY GENETICS
发明人:
GOMORD Veronique,FITCHETTE Anne Catherine,FAYE Loic
申请号:
US201715819537
公开号:
US2018105827(A1)
申请日:
2017.11.21
申请国别(地区):
美国
年份:
2018
代理人:
摘要:
A method for producing a recombinant protein in a plant, in particular a tobacco plant, preferably Nicotiana benthamiana, includes the following steps: a) culturing the plant aeroponically or hydroponically, preferably on mobile floats and under LED lighting; b) vacuum agroinfiltration of the plant obtained in a) by agrobacteria that include a DNA fragment coding for the recombinant protein; c) returning the plants to culture after step b), under the same conditions as for step a); and d) extracting and purifying the recombinant protein from the aerial portions of the plants produced in step c).
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