METHODE DE PRODUCTION DALLERGENES RECOMBINANTS DE HAUTE QUALITE PAR EXPRESSION TRANSITOIRE CHEZ NICOTIANA BENTHAMIANA

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专利权人:

ANGANY GENETICS

发明人:

VERONIQUE GOMORD,ANNE CATHERINE FITCHETTE,LOIC FAYE

申请号：

FR1255510

公开号：

FR2991996B1

申请日:

2012.06.13

申请国别(地区):

FR

年份:

2016

代理人:

摘要：

A method for producing a recombinant protein in a plant, in particular a tobacco plant, preferably Nicotiana benthamiana, includes the following steps: a) culturing the plant aeroponically or hydroponically, preferably on mobile floats and under LED lighting b) vacuum agroinfiltration of the plant obtained in a) by agrobacteria that include a DNA fragment coding for the recombinant protein c) returning the plants to culturing after step b), under the same conditions as for step a) and d) extracting and purifying the recombinant protein from the aerial portions of the plants produced in step c).

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