A manufacturing method of Antrodia cinnamomea improving extraction rate of dehydrosulphurenic acid is disclosed. The manufacturing method comprises: soaking a raw herb of Antrodia cinnamomea in a salt solution to obtain a soaked Antrodia cinnamomea and soaking the soaked Antrodia cinnamomea in a alcohol solvent, followed by ultrasonic vibration to obtain an Antrodia cinnamomea extract.