The invention relates to an apparatus for separating tissue cells from a fluid, with a collection container forming a collection space, which is connected, on one hand, with a negative pressure source and which is connected, on the other hand, with a supply line for the fluid-tissue cell mixture.It is provided that inside the collection space (18) a communicating tube (16) is arranged, which terminates at a distance from the bottom (17) of the collection container (14), wherein a connection is provided between the communicating tube (16) and the collection space (18) above a fluid-tissue cell mixture level (22) established in the collection container (14), and a suction element (20) which is connected with the negative pressure source (6) is arranged inside the communicating tube (16).