The invention discloses an edible composition as well as a preparation method and application thereof. The edible composition comprises inulin and butyric acid producing bacteria in a weight ratio of 1 to 1. Inulin and butyric acid producing bacteria in the edible composition have synergistic effects and can conduce to obviously increasing the concentrations of butyric acids in intestinal tracts of animals and the quantities of probiotics, such as lactobacillus and bifidobacterium, and inhibiting growth of harmful bacteria, thus effectively regulating intestinal floras of animals and then enhancing the immunities based on the effects of the intestinal floras and preventing or treating the diseases, such as obesity, diabetes mellitus, hypertension, hyperlipidemia or tumors.