The present invention relates to the multispectral imaging of samples, in particular of biological tissues. The invention further relates to a method for acquisition of fluorescence and reflectance images of an object (400) comprising the steps of alternatingly illuminating the object (400) with at least a first light having several spectral regions of high intensity, wherein the first light has at least one region of low intensity that is of longer wavelength to a region of high intensity, and at least a second light having at least one spectral region of high intensity, recording a first image of the object during illumination of the object with the first light and a second image of the object during illumination of the object with the second light using a common sensor array (200), wherein the light recorded by the sensor array (200) is attenuated in at least one of the spectral regions in which the first light has high intensities.
