The disclosure relates to the combined use of an immunotherapeutic composition comprising recombinant Listeria strains expressing a heterologous antigen fused to a truncated listeriolysin O (tLLO), a truncated ActA protein, or a PEST amino acid sequence and an antibiotic regimen, which may be sequentially administered in order to prevent the persistence, seeding of Listeria and/or formation of Listeria biofilms while allowing for an anti-tumor/ anti-cancer or anti infectious disease immunotherapeutic response to take place. Disclosed are also methods of inducing an immune response, and treating, inhibiting, or suppressing cancer or tumors comprising administering the above composition.本文揭示係有關於包含重組李斯特菌屬菌株表現異源抗原融合至截短李斯特菌溶胞素O(tLLO)、截短ActA蛋白、或PEST胺基酸序列的免疫治療性組成物及抗生素方案的組合用法，其可循序地投予以便防止李斯特菌屬的持續存在、播種及/或李斯特菌屬生物膜之形成，同時允許產生抗腫瘤/抗癌症或抗感染性疾病的免疫治療反應。也揭示包含投予前述組成物的誘導免疫反應，及治療、抑制、或遏止癌症或腫瘤之方法。
