Disclosed herein are recombinant Listeria strains comprising nucleotides encoding two or more heterologous antigens each fused to a truncated LLO, an N-terminal ActA or a PEST-sequence, methods of preparing same, and methods of inducing an immune response, and treating, inhibiting, or suppressing cancer or tumors comprising administering same.本文揭示重組李斯特菌屬菌株,其包含編碼兩種或多於兩種異源抗原之核苷酸,該等異源抗原各自與截短LLO、N端ActA或PEST序列融合;該等菌株之製備方法;及誘導免疫反應、及治療、抑制或遏制癌症或腫瘤之方法,該等方法包含投與該等菌株。
