Janne Brunstedt,Jens Frisbæk Sorensen,Jørn Borch Søe,Charlotte Johansen Vedel,Birgitte Ø. Wittschieben,Jørn Dalgaard Mikkelsen,Charlotte Horsmans Poulsen,Lene B. Jensen,Lone B. Miller,Morten K. Larsen
申请号：
US13444302
公开号：
US20130034627A1
申请日:
2012.04.11
申请国别(地区):
US
年份:
2013
代理人:
摘要：
The present invention provides a method for preparing a variant lipid acyltransferase enzyme by expressing a nucleotide sequence encoding a lipid acyltransferase which may comprise at least one modification at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120) and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6 in a host organism.
