The invention provides a method for monitoring the degree of protein denaturation and aggregation during the course of a heat treatment process comprising the steps of : heating an aqueous protein-containing fluid to a temperature corresponding to a heat treatment intensity in proximity to a previously established expected heat treatment intensity collecting a sample of the heated protein solution diluting said sample (s) in a buffer formulated so that: the pH in said sample is substantially unchanged, the ionic conditions in said sample are substantially unchanged, and the viscosity of said sample is at least substantially- maintained, all relative to the undiluted sample, measuring the turbidity of said sample relative to an untreated control sample comparing the turbidity measurement obtained with previously established turbidity measurement limits corresponding to a desired average protein aggregate size range collecting a sample of the heated protein solution mixing said sample (s) with a chemical -SH group reaction measurement system and comparing the SH-group measurement obtained with previously established SH-group measurement limits corresponding to a desired degree of denaturation.Linvention concerne un procédé servant à suivre le degré de dénaturation et dagrégation dune protéine au cours dun procédé de traitement thermique, comprenant les étapes consistant : à chauffer un fluide aqueux contenant une protéine à une température correspondant à une intensité de traitement thermique au voisinage dune intensité de traitement thermique attendue préalablement établie à recueillir un échantillon de la solution de protéine chauffée à diluer ledit échantillon (s) dans un tampon formulé de façon à ce que : le pH dudit échantillon ne soit sensiblement pas modifié, les conditions ioniques dans ledit échantillon ne soient sensiblement pas modifiées et la viscosité dudit échantillon soit au moins sensiblement maintenue, tout cela par rapport à léchantillon non dilué et
