The invention relates to an injectable amniotic membrane tissue graft product for percutaneous, minimally invasive, surgical, and topical therapy of injury and disease and methods of applying such tissue grafts to a subject at a particular location. The injectable preparations maximize and standardize available quantities of non-cellular biological compounds to enhance therapeutic efficacy. The tissue graft preparations are semi-viscous fluids with standardized properties which may be intraoperatively transplanted at the recipient site using a needless syringe, by non-operative percutaneous injection through a hypodermic needle, or direct topical application to open cutaneous wounds and external soft tissue defects.