МЕЙСОН Джастин Томас (US),ЛЕТТОУ Лесли Джеймс (US),ЭБИ Марк Алан (US),ЭБИ Уилльям Х. (US),ВЕЛЬЦ Гюнтер (DE),ВЕРХАГЕ Стивен (BE),ДЕ БЕККЕЛЕР Марк (BE),ХАБЕКС Верле (BE),ФЕРЮЛЛО Жан-Марк (FR)
申请号：
RU2012125961/10
公开号：
RU2012125961A
申请日:
2010.11.23
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
1. A method for identifying the simultaneous presence of the EE-GM3 and EE-GM1 elite events in biological samples, comprising detecting the region specific for EE-GM3 using a specific primer or probe that specifically recognizes the 5'- or 3'-flanking sequence of foreign DNA in EE-GM3, including herbicide resistance genes, and the detection of a region specific for EE-GM1, using a specific primer or probe that specifically recognizes the 5'- or 3'-flanking sequence of foreign DNA in EE-GM1, including genes resistant to herbicides. 2. The method according to claim 1, characterized in that it includes the amplification of two DNA fragments between 50 and 1000 bp in length. from a nucleic acid present in said biological sample using a first polymerase chain reaction using at least two primers, one of which recognizes the 5'-flanking region of foreign DNA in EE-GM3 containing herbicide resistance genes, said 5 ' the β-flanking region includes the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 1451 or the 3 ′ flanking region of foreign DNA in EE-GM3 containing herbicide resistance genes, said 3 ′ flanking region including the nucleotide sequence of the complementary chain of SEQ ID No 3 from nucleotide 241 to nucleotide 1408, and the other of these primers recognizes the sequence within the foreign DNA EE-GM3 including the nucleotide sequence of the complementary chain of SEQ ID No 2 from nucleotide 1452 to nucleotide 1843 or the nucleotide sequence of SEQ ID No. 3 from nucleotide 1 to nucleotide 240, or the other of these primers recognizes1. Способ идентификации одновременного присутствия элитных событий EE-GM3 и EE-GM1 в биологических образцах, включающий обнаружение области, специфической для EE-GM3, с помощью специфического праймера или зонда, специфически распознающего 5'- или 3'-фланкирующую последовательность чужеродной ДНК в EE-GM3, включающей гены устойчивости к гербицидам, и обнаружение области, специфической для EE-GM1, с помощью специфиче
