HANS PETER SCHWARZ,LEOPOLD BRUCKSCHWAIGER,THOMAS GUNDINGER,JULIA NRNBERGER,WOLFGANG TESCHNER
申请号：
ARP130100566
公开号：
AR090425A1
申请日:
2013.02.25
申请国别(地区):
AR
年份:
2014
代理人:
摘要：
Methods for preparing compositions of Blood Proteins from pooled plasma. In a way of realization, provides a Scheme of Fractionation with alcohol can significantly increase the performance of Blood Proteins Purified from a plasma sample.Pooled Plasma Fractionation method comprises a first step of precipitation with a high concentration of alcohol, low pH. Also provided are Pharmaceutical compositions with Therapeutic Blood Proteins.Claim 1: a method for preparing a composition enriched in immunoglobulins from plasma, wherein Said method comprises the steps of: (a) Co precipitate immunoglobulins and 1-antitrypsin (a1pi) from a Grouping of Cohn,In a first step of precipitation by the addition of ethanol to the grouping of Cohn to a final concentration of between 20% and 30% (V / V) at a pH of between 5.0 and 6.0, to form a First precipitate and a supernatant； (b) to solubilize the immunoglobulins in the first precipitate.To form a suspension that has a first portion that includes soluble and insoluble portion comprising an immunoglobulin a1pl； (c) separation of the soluble portion of the first suspension of the insoluble portion of the first suspension； and (d) to recover the soluble fraction of the pri Suspension, thus forming an Enriched Composition of immunoglobulins.Métodos para elaborar composiciones de proteínas sanguíneas a partir de plasma agrupado. En una forma de realización, se provee un esquema de fraccionamiento con alcohol que permite aumentar significativamente el rendimiento de proteínas sanguíneas purificadas a partir de una muestra de plasma de partida. Método de fraccionamiento de plasma agrupado que comprende un paso inicial de precipitación con una concentración elevada de alcohol, a pH bajo. También se proveen composiciones farmacéuticas con proteínas sanguíneas terapéuticas. Reivindicación 1: Un método para elaborar una composición enriquecida en inmunoglobulinas a partir de plasma, caracterizado porque dicho método comprende los pasos de: (a)
