The present invention relates to a method for manufacturing disinfectant comprising the steps of: (a) manufacturing an amino acid complex composition A by adding purified water to a mixture including nisin, sorbitan fatty acid ester, and glycerin fatty acid ester, and agitating the same (b) manufacturing an amino acid composition B by mixing L-lysine, polylysine, glycine, L-valine, sucrose fatty acid ester, lauryl amino dipropionate sodium, embryo extract, peony extract, and gallic acid, and adding the same to the amino acid complex composition A and agitating the same (c) manufacturing an amino acid complex C by mixing sodium glutamate, L-arginine. L-leucine, ctric acid, sodium Ethylenediamine-tetraacetate, and propylene glycol, and adding the same to the mixed solution of A and B and agitating the same (d) adding the purified water to the mixed solution and agitating the same and (e) fixing the mixed solution as in a state without vibration. The present invention provides the method for manufacturing disinfectant with high economics which: prevents generation of harmful chlorine gas or manufacturing of carcinogens which are considered the weakness of conventional chlorine based disinfectants and has no corrosiveness, thereby usable in any place. Moreover, the present invention uses natural ingredients with a food additive level, thereby retaining sterilizing power does not influence the flavor of food reserves, thereby being able to be used for washing vegetables and fruits and comprises a nonvolatile amino acid mixture as a main component, thereby keeping sterilizing power for a long time at room temperatures.COPYRIGHT KIPO 2015본 발명은, (a) 니신, 소르비탄지방산에스테르, 글리세린 지방산에스테르를 혼합한 혼합물에 정제수를 투입, 교반하여 복합 아미노산 조성물 A를 제조하는 단계 (b) L-리신, 폴리리신, 글리신, L-발린, 자당지방산에스테르, 라우릴아미노디프로피온산나트륨, 배아 엑기스, 목단 엑기스, 몰식자산을 혼합하여 복합 아미노산 조성물 B를 제조한 후, 이를 상기 복합 아미노산 조성물 A에 추가하여 교반하는 단계 (c) 글루타민산 나트륨, L-아르기닌, L-로이신, 구연산, 이.디.티.에이.이나트륨, 프로필렌글리콜을 혼합하여 복합 아미노산 조성물 C를 제조한 후, 이를 상기 A 및 B의 혼합액에 추가하여 교반하는 단
