The invention relates to preparations and methods of creating preparations of reconstituted amniotic membrane utilizing amniotic fluid, for use as combination tissue grafts in surgical and minimally invasive medical therapy of injury and disease. The preparations maximize available quantities of viable mesenchymal stem cells and non-cellular bioactive compounds to enhance therapeutic efficacy. The tissue graft preparations are semi-viscous fluids which may be intraoperatively transplanted at the recipient site using a needless syringe or by non-operative percutaneous injection through a hypodermic needle.
