AROUL SELVAM RAMADASS,ALEXANDRE AKOULITCHEV,ELIZABETH JANE MELLOR
申请号：
GB201021744
公开号：
GB2473392B
申请日:
2009.06.02
申请国别(地区):
GB
年份:
2013
代理人:
摘要：
The present invention relates to a method of monitoring epigenetic changes comprising monitoring changes in conditional long range chromosomal interactions at at least one chromosomal locus where the spectrum of long range interaction is associated with a specific physiological condition, the method comprising the steps of: &mdash；(i) in vitro crosslinking of said long range chromosomal interactions present at the at least one chromosomal locus； (ii) isolating the cross linked DNA from said chromosomal locus； (iii) subjecting said cross linked DNA to restriction digestion with an enzyme that cuts at least once within the at least one chromosomal locus； (iv) ligating said cross linked cleaved DNA ends to form DNA loops； (v) identifying the presence of said DNA loops； wherein the presence of DNA loops indicates the presence of a specific long range chromosomal interaction.
