ДЕ ФУЖЕРОЛЛЬ Антонин (US),РОЙ Атану (US),ШРАМ Джейсон П. (US),СИДДИКИ Сухаиб (US),ХАТАЛА Пол (US),БАНСЕЛЬ Стефан (US)
申请号：
RU2014117701/10
公开号：
RU2014117701A
申请日:
2012.10.03
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1. An isolated polynucleotide encoding a polypeptide of interest, wherein said isolated polynucleotide comprises: (a) a sequence of n numbers related nucleosides or nucleotides containing at least one modified nucleoside or nucleotide is compared with the chemical structure of the nucleoside or nucleotide A, G, U or C, (b) 5-UTR, comprising at least one sequence of Kozak, (c) 3 UTR, and (d) at least one structure 5-kepa.2. An isolated polynucleotide according to Claim. 1 further comprising a poly-A-chast.3 terminal. An isolated polynucleotide according to Claim. 1, which is ochischennym.4. An isolated polynucleotide according to Claim. 1, wherein at least one 5-cap structure selected from the group consisting of 0 the cap, the cap 1, ARCA, Inosine, N1-methylguanosine, 2-ftorguanozina, 7-deazaguanosine, 8-oxoguanosine, 2 aminoguanozina, LNA-guanosine and 2-azidoguanozina.5. An isolated polynucleotide according to Claim. 1, wherein the modification of the nucleoside is a nucleoside base and / or sugar moiety nukleozida.6. . An isolated polynucleotide according to claim 5, wherein the modification is a nucleotide base and nucleoside osnovanieimeet formula: gdeoboznachaet single or double bond X represents O or S each of U and W independently represents C or N V is O, S, C or N wherein when V is C, then R is H, Ci ^ alkyl, alkenyl, Calkinil, halogen or -OR, wherein each Ci ^ alkyl, alkenyl, Calkinila optionally substituted by -OH, -NRR, -SH, -C (O) R, -C (O) oR, -NHC (O) R or -NHC (O) oR and wherein when V represents O, S or N, then Rotsutstvue t R is H, -OR, -SR, halogen -NRRili ilikogda V is C, then r and together with the atoms1. Выделенный полинуклеотид, кодирующий представляющий интерес полипептид, причем указанный выделенный полинуклеотид содержит:(a) последовательность из числа n связанных нуклеозидов или нуклеотидов, содержащих по меньшей мере один модифицированный нуклеозид или нуклеотид по сравнению с химической структурой нуклеозида или нуклеотида A, G, U или
