Disclosed is an isolated antibody against human TROP-2, which has anti-tumour activity in vivo, wherein the amino acid sequences of CDR 1 to 3 of the H chain V region of the antibody are IYWIN, NIYPSDSYTNYNQKFKD and TSMADY, respectively, and the amino acid sequences of CDR 1 to 3 of the L chain V region of the antibody RASQSIGTSIH, YASESIS and QQSNSWPFT, respectively.