A pet grooming assembly includes a panel that may be coupled to a vertical support surface thereby facilitating the panel to be accessible to a pet. An adhesive layer is coupled to the panel. The adhesive layer may adhesively engage the vertical support surface. Thus, the panel is retained on the vertical support surface. A plurality of bristles is provided and each of the bristles is removably coupled to the panel. Thus, each of the bristles may frictionally engage the pet thereby facilitating the pet to groom themselves.