A dental operation-guiding structure includes a bite plate, a marking plate, and at least one connection frame connecting the bite plate and the marking plate. The bite plate is occluded by lower and upper teeth of a human body. The marking plate includes a plate body and at least one window penetrating the plate body. The plate body is adjacent to a tissue surface, and the windows defines a position to be operated. By occluding the dental operation-guiding structure, practical positions, patterns and ranges for the operation can be easily defined. The method for producing the dental operation-guiding structure overlaps a 3D bone image of a target object onto a 3D teeth-mold image thereof to obtain a reconstructed 3D image including clear contours of bones and soft tissues, bases on the reconstructed 3D image to design the dental operation-guiding structure, and finally produces the dental operation-guiding structure by 3D printing.
