PURPOSE: Culture liquid of human adipose-derived stem cells or concentrate liquid thereof is provided to effectively regenerate ectoderm-originated nerve cells and to treat various neural diseases.CONSTITUTION: A composition for promoting ectoderm-originated cell regeneration contains: stem cell culture liquid which is prepared by sub-culturing human adipose-derived stem cells in a serum medium and culturing in a serum-free medium and concentrate liquid thereof as an active ingredient. A method for preparing the culture liquid or concentrate liquid thereof comprises: a step of sub-culturing human adipose-derived stem cells in a serum medium and continuously culturing the stem cells in a serum-free medium a step of treating the stem cells by hypoxic culture, UV irradiation, low frequency treatment, nutrient deficiency, or mechanical friction a step of adding vitamin A, B, C, or D to the culture liquid and culturing the stem cells and a step of filtering the culture liquid.COPYRIGHT KIPO 2013본 발명은 인간 지방유래 줄기세포를 혈청배지에서 계대배양한 다음 무혈청배지에서 연속배양한 후, 여과를 하여 얻은 줄기세포 배양액 또는 그 농축액을 유효성분으로 함유하는 외배엽 기원 세포의 재생 촉진용 조성물, 상기 조성물을 손상 조직에 국소적으로 도포 또는 주사하는 것을 특징으로 하는 외배엽 기원 세포의 재생을 촉진하는 방법 및 상기 조성물을 유효성분으로 포함하는 신경질환 치료제에 관한 것이다.
