細胞をガラス化保存する方法および細胞のガラス化保存用容器
- 专利权人:
- 乾;裕昭
- 发明人:
- 乾 裕昭,水野 仁二,赤石 一幸,安齋 憲,栗城 瑛子,野口 香里,渡邉 百合
- 申请号：
- JP2010034885
- 公开号：
- JP5525280B2
- 申请日:
- 2010.02.19
- 申请国别(地区):
- JP
- 年份:
- 2014
- 代理人:
- 摘要：
PROBLEM TO BE SOLVED: To provide a cell preservation method that suppresses cytotoxicity by a cytoprotective liquid and increases a cell survival rate by efficiently and stably vitrifying and preserving cells by a very small volume of cytoprotective liquid.
SOLUTION: The method includes holding cells in &le50 nL volume of a preservation solution of a perfect non-serum (containing no serum component) having and preserving the cells in the presence of a refrigerating agent. Preferably, the cells are preserved in the presence of a refrigerating agent by using a cell protective liquid containing a cryoprotective agent having concentration of &le15%.
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- 来源网站：
- 中国工程科技知识中心
